RNASeq course

Contents:

  • Introduction
  • Experiment Design
  • A brief introduction to linux shell
  • RNA Seq raw data
  • Gapped Alignment
  • Measuring expression levels from RNA Seq data
  • De novo RNA Seq assembly
RNASeq course
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RNA Seq courseΒΆ

Contents:

  • Introduction
  • Experiment Design
    • Exercise
  • A brief introduction to linux shell
    • Navigating in the file system
    • Some more commands
    • Redirect and pipe
    • Exercises
  • RNA Seq raw data
    • MD5Sum
    • The fastq file format
    • Quality control
    • Quality trimming
    • Exercise
  • Gapped Alignment
    • Hands-on exercise
    • Mapping
    • Processing with SAMTOOLS
    • Data visualization
    • Exercise
  • Measuring expression levels from RNA Seq data
    • Expression values / Transcript abundance
    • Calculating expression values
    • Exercise
  • De novo RNA Seq assembly
    • Exercise
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© Copyright 2018, Burkhard Steuernagel.

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